What is the role of Next Generation Sequencing (NGS) in nonunion of orthopaedic cases?

Study Overview

The purpose of this study is to investigate the role of Next Generation Sequencing (NGS), a molecular method to detect bacterial DNA presence, in 1) diagnosis of infected nonunion compared to microbiological culture and 2) compare NGS signal with clinical outcome and fracture healing.

Study Description

Per standard of care preoperatively, all patients must have blood drawn to check for any infection. Preoperative radiographs and possible CT scans of the nonunion site will also we obtained in keeping with routine local standard of care. The study team will collect information relevant to the history of orthopaedic procedures from medical records and use it for this study.

Three tissue cultures will be obtained for each case as per routine standard of care. Further, additional tissue and intraoperative swabs from identical sites as the cultures will be sent for NGS. The NGS samples will be sent to MircroGenDx laboratory for sequencing bacterial DNA. The tissue used for NGS would normally be discarded at the time of surgery.


  • IRB Number: 1907158823
  • Research Study Identifier: 1907158823
  • Principal Investigator: Roman Natoli, MD

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If you need help finding a study or have any questions, please contact us at inhealth@iu.edu or by phone at (888) 264-0005.